Terje J
Norbrygg-medlem
Der lærte jeg igjen noe nytt,men det blir vel mye styr for min del å begynne med brygging på laboratorienivåTja, så du videoen?
Bryan som har videoen har 20 års erfaring som hjemmebrygging og er mikrobiolog av yrke. Oppbevaring under olje er vanlig, oljen fryser ikke før ved -30°c, så man risikerer ikke at iskrystaller bryter opp cellemembranen.
Selv tenker jeg å kjøpe Herdins Parafinolje med medisinsk renhetsgrad fra denne butikken.
Wikipedia - Cell culture:
Mineral oil of special purity is often used as an overlay covering microdrops of culture medium in petri dishes, during the culture of oocytes and embryos in IVF and related procedures. The use of oil presents several advantages over the open culture system: it allows for several oocytes and embryos to be cultured simultaneously, but observed separately, in the same dish; it minimizes concentration and pH changes by preventing evaporation of the medium; it allows for a significant reduction of the medium volume used (as few as 20 microlitres per oocyte instead of several millilitres for the batch culture); and it serves as a temperature buffer minimizing thermal shock to the cells while the dish is taken out of the incubator for observation.